Current Protocols in Cell Biology

图书介绍

   

Juan S. Bonifacino, Mary Dasso, Joe B. Harford, Jennifer Lippincott-Schwartz, Kenneth M. Yamada (eds.)
Current Protocols in Cell Biology
Copyright  2004 John Wiley & Sons
DOI: 10.1002/0471143030.cbfores00
ISBN: 0-471-24108-3
Online Posting Date: May, 2004

Developed by leading scientists in the field,  Current Protocols in Cell Biology is an essential reference for researchers who study the relationship between specific molecules and genes and their location, function and structure at the cellular level.
* includes nearly 1,000 detailed protocols carefully written by the foremost investigators in cell biology from all around the world.
* offers expert research tips and guidelines for isolating and culturing cells in two- or three-dimensional cultures, analyzing the morphology of the cell, and characterizing the functions of organelles and molecules in the cell and its environment.
* includes special appendices on the use of inhibitors and pharmacologic agents, protein motifs, absorption and emission maxima for common fluorophores, and more.

Current Protocols in Cell Biology provides methods for basic culture and fractionation of cells; microscopic localization and tracking; and analysis of biosynthesis, modification, and transport of specific molecules. In addition, the one-volume looseleaf manual offers more complex procedures involved in the determination of the role of intracellular and extracellular environment; molecular reconstitution of specific processes; molecular and temporal analysis of the cell cycle; investigation of intracellular and intercellular communications; and analysis of intact cells, organelles, and specific molecules. These topics are thoroughly explained with detailed protocols, critical parameters and troubleshooting guidelines. Extensive background information and authors' commentaries provide all the necessary information and guidance to facilitate successful results. Quarterly updates, filed into the looseleaf binder, keep you and your laboratory current with the latest developments in cell biology methodology.

Table of Contents

Foreword by Kai Simons
Preface
Contributors
1 Cell Culture
2 Preparation and Isolation of Cells
3 Subcellular Fractionation and Isolation of Organelles
4 Microscopy
4A Organelle Atlas
5 Characterization of Cellular Proteins
6 Electrophoresis and Immunoblotting
7 Protein Labeling and Immunoprecipitation
8 Cell Cycle Analysis
9 Cell Adhesion
10 Extracellular Matrix
11 In Vitro Reconstitution
12 Cell Motility
13 Organelle Motility
14 Signal Transduction: Protein Phosphorylation
15 Protein Trafficking
16 Antibodies as Cell Biological Tools
17 Macromolecular Interaction in Cells
18 Cellular Aging and Death
19 Whole Organism and Tissue Analysis
Appendices
Index

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