[PDF] Lipid Analysis: Isolation, Separation, Identification and Lipidomic Analysis, 4th edition

ISBN: 0955251249

Category: Tutorial


Posted on 2017-12-29, by luongquocchinh.

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Author: William Christie, Xianlin Han | Category: Biology | Language: English | Page: 1 | ISBN: 0955251249 |

Description: This well-known and highly successful book was first published in 1973 and has been completely re-written in subsequent editions (published in 1982 and 2003). This new Fourth Edition has become necessary because of the pace of developments in mass spectrometry of intact lipids, which has given recognition of lipid analysis and lipidomics as a distinct science. To bring the book up to date with these developments, author William W. Christie is joined by co-author Xianlin Han. Although devoting considerable space to mass spectrometry and lipidomics, Lipid analysis remains a practical guide, in one volume, to the complexities of the analysis of lipids. As in past editions, it is designed to act as a primary source, of value at the laboratory bench rather than residing on a library shelf. Contents: Part 1 introducing lipids and their analysis: Lipids: Their structures and occurrence Chromatographic analysis of lipids: general principles Lipid extraction, storage and sample handling. Part 2 chromatographic separation and analysis of individual lipid classes: Analysis of simple lipid classes Chromatographic analysis of phospholipids and glycosyldiacylglycerols Chromatographic analysis of sphingolipids. Part 3 analysis of fatty acids: Preparation of derivatives of fatty acids Gas chromatographic analysis of fatty acid derivatives Isolation of fatty acids and identification by spectroscopic and related techniques. Part 4 chromatographic analysis of molecular species of lipids: Molecular species of triacylglycerols, diacylglycerols derived from complex lipids, and related lipids Chromatographic analysis of molecular species of intact phospholipids and glycolipids Positional distributions of fatty acids in glycerolipids. Part 5 mass spectrometric analysis of lipids in lipidomics: Introduction to mass spectrometric analysis of lipids in lipidomics Characterization of lipids by electrospray ionization mass spectrometry Practical identification of individual lipid species in lipid extracts of biological samples Quantification of lipid molecular species by electrospray ionization mass spectrometry.

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